BIOTECNOLOGIE MICROBICHE E AGROALIMENTARIModule GENETICA DEI MICRORGANISMI
Academic Year 2026/2027 - Teacher: CINZIA CAGGIAExpected Learning Outcomes
The course will provide the fundamentals for understanding, observing, interpreting, and managing the growth of microorganisms in complex matrices, whether food-related or environmental. It will also provide the basis for selecting, identifying, and characterizing microorganisms used in the agri-food sector. Independent judgment will be fostered through practical examples and case studies. Communication and learning skills will be encouraged through the preparation and presentation of scientific reports and/or laboratory work.
Specifically, students are expected to acquire understanding, communication skills, and independent judgment as outlined below:
Knowledge and understanding – (Dublin 1)
By the end of the course, students must demonstrate knowledge and understanding of the course's core content.
ASSESSMENT METHOD: Oral exam.
Applying knowledge and understanding – (Dublin 2)
By the end of the course, students must demonstrate the ability to identify the main natural genetic recombination phenomena in microorganisms of interest to the agri-food sector.
ASSESSMENT METHOD: Oral exam.
Autonomy of judgment – (Dublin 3)
By the end of the course, students must demonstrate the ability to form interpretive judgments based on the knowledge acquired; they must also be able to evaluate and interpret scientific literature.
ASSESSMENT METHOD: Oral exam.
Communication skills – (Dublin 4)
By the end of the course, students must demonstrate the use of correct technical terminology, adapting their oral and written communication to the required standard and specific objectives.
ASSESSMENT METHOD: Oral exam.
Lifelong learning skills – (Dublin 5)
By the end of the course, the student must demonstrate knowledge of the means and tools for keeping their knowledge and skills up to date and for undertaking further studies with a high degree of autonomy.
ASSESSMENT METHOD: In part, through an oral exam.
Course Structure
The teaching includes 21 hours of frontal lessons and 42 hours of exercises.
Teaching could be carried out in a mixed mode, also remotely, if required changes could be introduced with respect to previous statements, in line with the planned programme.
The planned activities concern practical exercises, laboratory tests, guided tours, in-depth seminars, group work. If the teaching is given in a mixed or remote way, the necessary changes with respect to what has been declared may be introduced, in order to comply with the planned program.
To guarantee equal opportunities and in compliance with the laws in force, interested students can ask for a personal interview in order to plan any compensatory and / or dispensatory measures, based on the didactic objectives and specific needs. For this purpose, it is also possible to contact the CInAP (Center for Active and Participatory Inclusion - Services for Disabilities and / or SLDs) of our Department, Prof. Anna De Angelis.
Required Prerequisites
Attendance of Lessons
Strongly reccomanded even if not mandatory.
Detailed Course Content
Microorganisms of biotechnological interest. Microorganisms as a "cell factory" for the production of goods and services. Research and development in industrial processes screening and optimization of production processes. Environmental issues and applications of microorganisms to protect the environment. Genetic improvement of microorganisms used in food industry. Development of microbial starters for the optimization of food fermentation. Basic concepts of bacterial genetics and effects of recent discoveries on the development of biotechnology.
Contribution of teaching to the goals of the 2030 Agenda for Sustainable Development. In particular, during the course, the issues related to the use of microorganisms as a strategy for achieving Goal 2: eradicate hunger in the world, achieve food security, improve nutrition and promote sustainable agriculture will be explored.
Textbook Information
The lecturer provides the materials through the STUDIUM platform (studium.unict.it). For the study of the discipline, the student can consult any text in the field.
| Author | Title | Publisher | Year | ISBN |
|---|---|---|---|---|
| ----- | Brock. Biologia dei microrganismi. Microbiologia generale, ambientale e industriale | Ediz. Mylab. | 2022 | 8891906298 |
| Stefano DonadioGennaro Marino | Biotecnologie microbiche | Casa Editrice Ambrosiana | 2008 | 978-8808184382 |
Course Planning
| Subjects | Text References | |
|---|---|---|
| 1 | Bacterial genetics and biotechnologies. Microrganisms as cell factories. Microbial exploitation in biotech. Some cases. | Lesson's materials.Text n. 1 |
| 2 | The microbial world and taxonomic procedures.Prokaryotic and eukaryotic cells. Microbial diversity as biotechnological resource. | Lesson's materials. Text n. 2 |
| 3 | * Genetic variability. the mutations. Ames test. Fluctuations test. Replica plating. Mutations, mutagens, and mutants. | Lesson's materials. Text n. 1. |
| 4 | Griffith's experiment. Transformation. | L |
| 5 | Genetic variability. DNA transpositions. Conjugation, transformation and transduction in bacteria. | Lesson's materials. Text n. 1. |
| 6 | Gene expression control. Operons. The lactose operon and the tryptophan operon in Escherichia coli. | Lesson's materials. Text. n. 1 |
| 7 | Yeasts and their use in biotechnology. Saccharomyces cerevisiae. Homotallism and heterothallism. | Lesson's materials. Text. n. 1. |
| 8 | Laboratory experiences. Choice of a natural matrix. Counting, isolation and identification of the main microbial species. Phenotypic and genotypic carachterization. |
Learning Assessment
Learning Assessment Procedures
The student's preparation will be evaluated based on the following criteria: level of knowledge and deepening of the topics, properties of language and exposure, and reasoning skills developed by the student.
The final mark will be the average of the votes obtained in the single module, according to the following structure:
Unsuitable
Topic knowledge and understanding: Significant deficiencies.
Significant inaccuracies
Analysis and synthesis skills: Irrelevant. Frequent generalizations. Inability to synthesize
Using references: Completely inappropriate
18-20
Topic knowledge and understanding: Threshold level. Obvious imperfections
Analysis and synthesis skills: Barely sufficient capacity
Using references: Just appropriate
21-23
Topic knowledge and understanding: Routine knowledge
Analysis and synthesis skills: capacity of correct analysis and synthesis. Argues logically and coherently
Using references: Use standard references
24-26
Topic knowledge and understanding: Good knowledge
Analysis and synthesis skills: Has good analytical and synthesis skills. Topics are expressed coherently
Using references: Use standard references
27-29
Topic knowledge and understanding: Detectable neat knowledge
Analysis and synthesis skills: Has remarkable analytical and synthesis skills
Using references: Has delved into the topics
30-30L
Topic knowledge and understanding: Evident general knowledge
Analysis and synthesis skills: Has remarkable analytical and synthesis skills
Using references: Significant insights
Examples of frequently asked questions and / or exercises
Example of questions: genetic variability in bacteria; mutations; fluctuation tests; homothallism and heterothallism in Saccharomyces cerevisiae; operons; phages.