GENETIC IMPROVEMENT OF FRUIT CROPS AND SUSTAINABLE BIOTECHNOLOGIES

Provide in-depth and specialized knowledge on the application of traditional breeding methods and modern biotechnologies in the fruit production sector. In particular, skills will be provided on the interventions that can be carried out according to the biological specifications of the individual fruit species, applied to in vitro cultivation, genetic engineering and structural fruit genomics.

In detail, with reference to the so-called “Dublin Descriptors”, the course aims to provide the following knowledge and skills.

Knowledge and understanding: The student will acquire specific knowledge on biotechnologies applied to the genetic improvement of fruit species, genome editing techniques and assisted selection with molecular markers.

Ability to apply knowledge and understanding: Lo will be able to produce students and accept genetic data to be used in plans for the improvement, management and preservation of fruit species in the Mediterranean area;

Autonomy of judgment (making Judgments): The knowledge and skills acquired during the course and in particular the individual study of some literature, and the critical analysis of literature will allow the student to achieve autonomy of judgment and ability to study and to operate independently.

Communication skills (communication skills): Lectures, other activities and individual study on selected teaching material will allow the student to acquire the basics of technical language, also in English. The student will be stimulated to use technical language in the relevant classroom, as well as during the other activities. The property of technical language contributes to the final evaluation of the student.

Learning skills (learning skills): During the course, the activity of collecting and examining scientific literature and writing scientific reports is aimed at preparing the student for self-employment and critical analysis of literature. These skills are useful for the preparation of the degree thesis, for the drafting of a research project for access to the doctorate and for the continuous updating of the knowledge and skills necessary for the profession.

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Riquadri laterali

Lectures with comparison and learning tests in the classroom (28 hours), laboratory exercises for in vitro culture, genetic transformation, application of molecular markers, bioinformatics analysis (28 hours).

If the teaching is given in a mixed or remote mode, the necessary changes may be introduced with respect to what was previously stated, in order to comply with the program envisaged and reported in the syllabus.

To guarantee equal opportunities and in compliance with the laws in force, interested students can ask for a personal interview in order to plan any compensatory and / or dispensatory measures, based on the didactic objectives and specific needs.

It is also possible to contact the referent teacher CInAP (Center for Active and Participatory Integration - Services for Disabilities and / or SLD) of our Department.

The reproductive biology of fruit trees

Peculiarities of tree biology

From classic to modern breeding

In vitro cultures for nursery and genetic improvement of fruit trees

Conservation of fruit germplasm

Somaclonal variation and in vitro selection

Genetic engineering: applications and prospects of use for transgenesis, cisgenesis and genome editing for fruit plants

Biochemical and molecular markers in the identification of genotypes, in phylogeny studies, in the creation of genetic maps, in the identification of genes for tree plants

Applied bioinformatics for useful traits for tree plants

Genetic improvement objectives for the main fruit species

1) Sansavini et al 'Arboricoltura generale' Pàtron Editore (per il miglioramento genetico tradizionale)

2) Rosa Rao e Antonietta Leone "Biotecnologie e Genomica delle Piante" Idelson-Gnocchi Editore oppure il

Barcaccia G., Falcinelli M. “Genetica e genomica” vol II e III. Liguori editore

3) Appunti del docente

4) Articoli monografici su riviste di settore indicati durante il corso

Altri testi utili: Approfondimenti su Galbiati et al., 'Biotecnologie sostenibili' Edagricole Editore

Oral examination. In particular, the relevance of the answers to the questions asked, the quality of the contents, the ability to connect with other topics covered by the program, the ability to report examples, the technical language properties and the overall expressive ability of the student will be assessed.

Verification of learning can also be carried out electronically, should the conditions require it.

Learning assessment may also be carried out on line, should the conditions require it.

Verification of learning takes place through an oral interview. The evaluation of the student's preparation will take place on the basis of the following criteria: learning ability and level of depth of the topics covered, properties of synthesis and exposition, and the student's ability to reason.

The vote follows the following scheme:

Unsuitable

Knowledge and understanding of the topic: Important shortcomings. Significant inaccuracies

Analysis and synthesis skills: Irrelevant. Frequent generalizations. Inability to synthesize

Use of references: Completely inappropriate

18-20

Knowledge and understanding of the topic: At the threshold level. Obvious imperfections

Ability to analyze and synthesize: Just enough skills

Use of references: As appropriate

21-23

Knowledge and understanding of the topic: Routine knowledge Analysis and synthesis skills: It is capable of correct analysis and synthesis. Argue logically and consistently

Use of references: Use standard references

24-26

Knowledge and understanding of the topic: Good knowledge

Analysis and synthesis skills: Has good analysis and synthesis skills. The arguments are expressed consistently

Use of references: Use standard references

27-29

Knowledge and understanding of the topic: Knowledge more than good

Ability to analyze and synthesize: He has considerable abilities of analysis and synthesis

Use of references: Has explored the topics

30-30L

Knowledge and understanding of the topic: Excellent knowledge

Ability to analyze and synthesize: He has considerable abilities of analysis and synthesis.

Use of references: Important insights.

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Riquadri laterali

Peculiarities of fruit breeding

Classification and applications of molecular markers for the genetic improvement of fruit trees

Applications of in vitro culture for tree plants

Genetic transformation mediated by explants of plant origin (arboreal plants)