BIOMOLECULAR METHODS
Academic Year 2017/2018 - 1° YearCredit Value: 8
Scientific field: AGR/07 - Agricultural genetics
Taught classes: 42 hours
Term / Semester: 1°
Learning Objectives
To provide students the theoretical and practical knowledge of the main techniques used to analyze the structure and function of biomolecules, in particular those that led to the development of new disciplines such as genomics, transcriptomics and proteomics.
Detailed Course Content
The manipulation of nucleic acids. Southern and Northern blots. Molecular and genomic markers. Qualitative and quantitative PCR. Cloning techniques and vectors. The genetic transformation of prokaryotes. Preparation and analysis of cDNA and genomic libraries. The DNA microarray technology. Subtractive hybridization. Methods of nucleic acid sequencing. RNAseq. The genetic transformation of eukaryotes. Cisgenesis and Intragenesis. Genome editing. Expression Vectors and fusion proteins. The recombinant DNA technology in the food industry.
Textbook Information
1) Biochimica e biologia molecolare: Principi e Tecniche, Wilson K., Walker J., ed. Raffaello Cortina (sesta edizione).
2) Rao R., Leone A., Biotecnologie e genomica delle piante, Idelson-Gnocchi ed.
3) Articoli scientifici forniti dal docente