MOLECULAR BIOTECHNOLOGY

Academic Year 2020/2021 - 1° Year
Teaching Staff: Angela Roberta Lo Piero
Credit Value: 8
Scientific field: AGR/07 - Agricultural genetics
Course Language: Italian
Taught classes: 42 hours
Exercise: 28 hours
Term / Semester:

Learning Objectives

To provide students the theoretical and practical knowledge of the main techniques used to analyze the structure and function of biomolecules, in particular those that led to the development of new disciplines such as genomics, transcriptomics and proteomics.


Course Structure

Teaching (8 CFU) includes 42 hours of frontal lessons and 28 hours of laboratory exercises. For lectures, the teacher will make use of power point presentations and videos, also in English. If the teaching is given in a mixed or remote mode, the necessary changes will be introduced, in order to respect the program reported here. Learning assessment might also be carried out remotely, should the conditions related to Covid-19 epidemiological emergency require it.


Detailed Course Content

The manipulation of nucleic acids. Southern and Northern blots. Molecular and genomic markers. Qualitative and quantitative PCR. Cloning techniques and vectors. The genetic transformation of prokaryotes. Preparation and analysis of cDNA and genomic libraries. The DNA microarray technology. Subtractive hybridization. Methods of nucleic acid sequencing. RNAseq. The genetic transformation of eukaryotes. Cisgenesis and Intragenesis. Genome editing. Expression Vectors and fusion proteins. The recombinant DNA technology in the food industry.


Textbook Information

1) Biochimica e biologia molecolare: Principi e Tecniche, Wilson K., Walker J., ed. Raffaello Cortina (ottava edizione).

2) Rao R., Leone A., Biotecnologie e genomica delle piante, Idelson-Gnocchi ed.

3) Scientific articles provided by the teacher